Haemoglobin analysis:
This test detects the anemia disease which have symptoms as dizziness , headache , hypotension and hair falling .This disease followed by nutrition instructions as:
-Eating meats without fats , vegetables , apple …etc .
-Partaking of vitamin c sources (natural preferred) after meal directly which helps in more absorption of iron in food that taken .
-Can partaking of coffee & tea but after meal by 2 hrs.
Procedure :
>Blood sample : fresh blood , EDTA blood or heparinized blood .
>Reagent : must be prepared according to kits of the chemicals used , and must stored in dark containers (this reagents breaks down by light) .
1. Put 2.5 ml of pre prepared working reagent in a test tube then put 10 µ blood .
2. Leave it for 5 minutes the read at wave length 546.
Calculation :
Reading x 36.77 = ……gm. X 6.9 = ……% .
Normal :
Adult male 14 – 16 gm
Adult female 12 – 14 gm
New born 18 – 21.5 gm
6 months 12.8 – 16 gm
1 year 10 – 14 gm
8 year 11.5 – 14.8 gm
14 year 11.6 – 15 gm
Abnormal :
-Less than normal values indicates the presence of anemia disease.
-The blood mesentration in females may cause decrease in hemoglobin levels.
-More than normal levels occurs in smoker men or in case of cancer in spleen (in this case must withdraw blood to decrease hemoglobin to normal levels).
Note:
in case of very low level of hemoglobin ( < 45%) indicates the bean anemia (breaking down of RBC’s usually by genetic cause) which affected by eating bean or any grains have the same species .
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